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发布网友
发布时间：2022-04-22 09:21
共2个回答
热心网友
时间：2023-10-25 05:00
Objective: To β △ thyroid hormone receptor gene in rats in the distribution of different characteristics, and is familiar with using PT-PCR analysis of the results. Method: TR β △ gene were expressed in a variety of organizations, including the liver, brain, skeletal muscle, heart, kidneys express the most. From various organizations in the mRNA, to extract the mRNA as a template retroviral cDNA synthesis of the first chain. And the template for PCR amplification TR β △ double-stranded cDNA. According to the purpose of gene transcription design of the two primers. Using fluorescence quantitative PCR method of quantitative analysis. Results: The Organization of extracting RNA, OD260/OD280 are between 1.7-2.0 between, OD260/230 range between 1.5-1.9. Identification of agarose gel electrophoresis of PCR results showed that in the expected around about 160 bp PCR procts with a purpose. TR βΔ PCR proct of the identification of agarose gel electrophoresis results showed that the PCR amplification of DNA molecular size to 176 bp for the purpose of the zone. Manager gene and gene quantitative determination of its amplification curve and no dissolution of miscellaneous peak, while the corresponding peak melting song Tm value and amplified procts similar to the theory of Tm. Conclusion: The results showed that TR β △ gene expression at the mRNA level of a certain spatial distribution of characteristics: the liver, brain, spleen, kidney tissue in both the gene expression, the expression and spleen was significantly higher than that of other organizations.
热心网友
时间：2023-10-25 05:01
Goal: Understood the thyroxine acceptor β△ gene in big mouse different organization distributed characteristic, is familiar with with PT-PCR analyzes the result. Method: TRβ△ the gene has the expression in many kinds of organizations, in which liver, the brain, the skeleton muscle, the heart, the kidney expression are most. Withdraws big mouse many place organizations mRNA, withdraws mRNA is the template counter plication synthesizes the cDNA first chain. And expands take it as template PCR increases the goal gene which TRβ△ the double strand cDNA. basis copies to design two to direct the thing. Uses fluorescence quota PCR the method to carry on the quantitative analysis. Finally: The extraction organizes total RNA, OD260/OD280 to be situated between between 1.7-2.0, OD260/230 is situated between between 1.5-1.9. The agarose gel electrophoresis appraises PCR to expand increases the result to demonstrate that, has the goal about anticipated 160bp to expand approximately increases the proct belt .TRβΔ PCR proct the agarose gel electrophoresis appraisal result to indicate, expands the DNA molecular weight size after PCR which increases is the 176bp goal banding. The goal gene and the steward gene quantitative determination, it expands increases the curve and the curve of solubility has not seen the mixed peak, simultaneously melts the tune prominent peak correspondence Tm value with to expand increases the proct theory Tm to be close. Conclusion: The findings showed TRβ△ the gene has certain spatial distribution characteristic in the mRNA level expression: The liver, the brain, the spleen, in the kidney organization has this gene expression, also in the spleen expression quantity is higher than other organizations obviously.
